ldl-particles labeled with dil fluorescence dye Search Results


anti ldlr antibody  (Novus Biologicals)
90
Novus Biologicals anti ldlr antibody
<t>LDLR</t> colocalizes with calnexin-ER in FH HLC. (A) After differentiation, HLC were treated overnight in LPDS medium supplemented with rosuvastatin followed by immunocytochemistry and confocal imaging, 100× oil immersion objective. AMIRA software was used to stack slices and merge channels to present an overview of localization of the LDLR (green), calnexin-ER (red) and nucleus (DAPI). (B) Quantification of colocalization using Fluoview software indicated that NC-HLC did have a significantly greater colocalization of the LDLR with calnexin than C-HLC. The graph values represent the mean±s.d. ( n =5) per cell type from three experiments repeated in the laboratory. Statistics were performed using an unpaired two-tailed Student's t -test, **P <0.01. (C) Both non-corrected (NC) and corrected (C) cell lines were differentiated to HLC and assayed for surface LDLR expression <t>using</t> <t>AF647-conjugated</t> anti-LDLR antibody clone C7 (top). Cells were quantified for percentage total positive labeling (middle) and mean fluorescence intensity (MFI) (bottom).
Anti Ldlr Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldl-particles+labeled+with+dil+fluorescence+dye/LDLR+Antibody+(C7)/pmc07157586-270-8-12
Average 90 stars, based on 1 article reviews
anti ldlr antibody - by Bioz Stars, 2026-09
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LDLR colocalizes with calnexin-ER in FH HLC. (A) After differentiation, HLC were treated overnight in LPDS medium supplemented with rosuvastatin followed by immunocytochemistry and confocal imaging, 100× oil immersion objective. AMIRA software was used to stack slices and merge channels to present an overview of localization of the LDLR (green), calnexin-ER (red) and nucleus (DAPI). (B) Quantification of colocalization using Fluoview software indicated that NC-HLC did have a significantly greater colocalization of the LDLR with calnexin than C-HLC. The graph values represent the mean±s.d. ( n =5) per cell type from three experiments repeated in the laboratory. Statistics were performed using an unpaired two-tailed Student's t -test, **P <0.01. (C) Both non-corrected (NC) and corrected (C) cell lines were differentiated to HLC and assayed for surface LDLR expression using AF647-conjugated anti-LDLR antibody clone C7 (top). Cells were quantified for percentage total positive labeling (middle) and mean fluorescence intensity (MFI) (bottom).

Journal: Disease Models & Mechanisms

Article Title: Familial hypercholesterolemia class II low-density lipoprotein receptor response to statin treatment

doi: 10.1242/dmm.042911

Figure Lengend Snippet: LDLR colocalizes with calnexin-ER in FH HLC. (A) After differentiation, HLC were treated overnight in LPDS medium supplemented with rosuvastatin followed by immunocytochemistry and confocal imaging, 100× oil immersion objective. AMIRA software was used to stack slices and merge channels to present an overview of localization of the LDLR (green), calnexin-ER (red) and nucleus (DAPI). (B) Quantification of colocalization using Fluoview software indicated that NC-HLC did have a significantly greater colocalization of the LDLR with calnexin than C-HLC. The graph values represent the mean±s.d. ( n =5) per cell type from three experiments repeated in the laboratory. Statistics were performed using an unpaired two-tailed Student's t -test, **P <0.01. (C) Both non-corrected (NC) and corrected (C) cell lines were differentiated to HLC and assayed for surface LDLR expression using AF647-conjugated anti-LDLR antibody clone C7 (top). Cells were quantified for percentage total positive labeling (middle) and mean fluorescence intensity (MFI) (bottom).

Article Snippet: Cells were then labeled with Alexa Fluor 647-conjugated anti-LDLR antibody (C7 clone; Novus Biologicals) or mouse IgG 2b isotype control (Novus Biologicals) according to the manufacturer's instructions (1 h at 4°C, protected from light) or left unlabeled.

Techniques: Immunocytochemistry, Imaging, Software, Two Tailed Test, Expressing, Labeling, Fluorescence